Enzyme Substrate Detection Reagent

Character This product is white or light yellow granules

Clarification degree Colorless or slightly yellow

pH 7.0~7.6

weight 2.7±0.5g

Storage Long term storage,refrigerating,drying,sealing and avoiding light storage at 4~8℃。

Term of validity 1 years

Enzyme Substrate Detection Reagent Working principle

In the water samples containing total coliform bacteria,the target bacteria were cultured in the Minimal Medium 0NPG-MUG medium at 36±1 C.The specific enzyme beta galactosidase produced by the total coliform bacteria can decompose the color source substrate of the Minimal Medium 0NPG-MUG medium,which makes the culture medium yellow;meanwhile,the large intestine in the water sample is in large intestine.Escherichia coli produces a specific beta glucuronase to decompose the fluorescent substrate MUG in Minimal Medium 0NPG-MUG medium and produce characteristic fluorescence.The same principle,the heat coliform group(fecal coliform group)will decompose the color source substrate ONPG in the Minimal Medium 0NPG-MUG medium at 44.5±0.5 C,making the medium yellow.

Packaging specification 200/box

Usage method

Qualitative detection

Setp 1 Add reagent in 100 ml water sample,dissolve it.and Culture 24 h at 36±1℃

Step 2 Read result

Colorless=negative

Yellow=total coliform positive

Yellow=total coliform positive

Yellow+fluorescence=Escherichia coli positive

Quantitative detection

Note:the heat tolerant coliform group(fecal coliform)needs

to be cultured at 44.5±0.5℃24 h,and yellow is positive.

Quantitative detection

Step 1 Add reagent in 100 ml water sample,dissolve it.

Step 2 Pour into the 51 or 97 detection plate.

Step 3 Use LK sealer to seal the detection plate,and cultivate 24 h at 36℃

Step 4 Results qualitative test was used to check the MPN table count.

Note:the heat tolerance coliform group(fecal coliform group)needs to be cultured at 44.5±0.5℃24 h,and the Yellow pore pattern is positive,and the corresponding MPN table count is found.