1.Light Source System

(1)Visible Light Source:Utilizes ultra-bright tri-color LED hybrid light sources,comprising 4 white light and 4 warm light sources.Light sources are directly controllable via software.The light assembly contains 144 imported low-voltage LED chips,offering high brightness,low energy consumption,soft light without flicker,stable low light decay,and extended lifespan.

(2)★Ultraviolet Light Source:Includes 2 narrow-band 365nm UV LED light sources.

(3)All 8 visible light channels and 2 UV light channels are software-controllable.The software allows independent adjustment of each channel's intensity.Adjusted parameters can be saved to methods for direct recall in subsequent sessions.

(4)★Fully Enclosed Steel Photography Chamber:Interior walls feature black matte coating to eliminate reflections,glare,and light spots.Excellent sealing effectively blocks interference from stray light sources.

2.Instrument Parameters

(1)Countable Parameters:Total Colony Count,Total Coliforms,Fecal Coliforms,Escherichia coli,Enterococcus,Pseudomonas aeruginosa,etc.

(2)Sample Types:51/97-well quantitative plates,84-well quantitative plates(dedicated for total colony count enzyme substrate method),90mm petri dishes,45mm filter membranes,etc.;

(3)Fluorescent Bacteria Detection:Supports detection of fluorescent bacteria like Escherichia coli under 365nm UV light;

(4)Door:Self-closing drawer door;

(5)Sample Trays:Compatible with multiple sample types,including 51/97-well quantitative plates,84-well quantitative plates(dedicated for total colony count enzyme substrate method),90mm petri dishes,45mm filter membranes,etc.;

(6)Data Interface:Dust-proof independent USB 3.0 port.

3.Counting and Analysis Software

(1)Analysis Items

Total(fecal)coliforms,Escherichia coli,Enterococcus,Pseudomonas aeruginosa,Total bacteria count-enzyme substrate method,Total bacteria count-plate count method,Total bacteria count-filter membrane method

(2)Basic Counting Functions

One-click intelligent counting:Yellow 51-well counting,Yellow 97-well counting,Fluorescent 51-well counting,Fluorescent 84-well counting,Fluorescent 97-well counting,Plate method counting,Filter membrane method counting;

(3)Detailed Functions of Total(Fecal)Coliforms Module:

★Image Display:Includes zoom in,zoom out,and image panning;

★One-Click Statistics:Yellow 51-well-Total(Fecal)Coliforms,Yellow 97-well-Total(Fecal)Coliforms;

★Manual Correction:Select-and-add counting,count reversal,single-click cell deletion,area deletion;

★Quick functions:Restore original image,save,export PDF,export Excel;

★Result display:Positive well count,large well positive,small well positive,MPN,upper 95%confidence interval,lower 95%confidence interval;

★Annotation:Text input,annotation undo;

★Instrument settings:General settings and lighting settings.

(4)Detailed Functions of the Escherichia coli Module:

★Image Display:Includes zoom in,zoom out,and image panning;

★One-Click Statistics:51 Fluorescence-Escherichia coli,97 Fluorescence-Escherichia coli;

★Manual Correction:Yellow-Total coliform comparison,box selection for count addition,count undo,single-click box deletion,area deletion;

★Quick Functions:Restore Original Image,Save,Export PDF,Export Excel;

★Result Display:Positive Well Count,Large Well Positive,Small Well Positive,MPN,Upper 95%CI Limit,Lower 95%CI Limit;

★Annotation:Text Input,Annotation Undo;

★Instrument settings:General settings and lighting settings.

(5)Enterococcus module detailed functions:

★Image display:Includes zoom in,zoom out,and image panning;

★One-click statistics:51 Fluorescent-Enterococcus,97 Fluorescent-Enterococcus;

★Manual correction:Add count via selection,count undo,single-click box deletion,area deletion;

★Quick functions:Restore original image,save,export PDF,export Excel;

★Result display:Positive well count,large well positive,small well positive,MPN,upper limit of 95%confidence interval,lower limit of 95%confidence interval;

★Annotation function:Text input,annotation undo;

★Instrument Settings:General settings and lighting settings.

(6)Pseudomonas aeruginosa Module Detailed Functions:

★Image Display:Includes zoom in,zoom out,and image panning;

★One-Click Statistics:51 Fluorescence-Pseudomonas aeruginosa,97 Fluorescence-Pseudomonas aeruginosa;

★Manual Correction:Add counts via selection,count undo,single-click box deletion,area deletion;

★Quick functions:Restore original image,save,export PDF,export Excel;

★Result display:Positive well count,large well positive,small well positive,MPN,upper 95%confidence interval,lower 95%confidence interval;

★Annotation function:Text input,annotation undo;

★Instrument Settings:General settings and lighting settings.

(7)Total Bacteria Count-Enzyme Substrate Method Module Detailed Functions:

★Image Display:Includes zoom in,zoom out,and image panning;

★One-Click Statistics:84 Fluorescence-Total Colony Count;

★Manual Correction:Add count via selection,count undo,single-click box deletion,area deletion;

★Quick functions:Restore original image,save,export PDF,export Excel;

★Result display:Number of positive wells,MPN,upper limit of 95%confidence interval,lower limit of 95%confidence interval;

★Annotation function:Text input,annotation undo;

★Instrument settings:General settings and lighting settings.

(8)Total Bacteria Count-Plate Count Method Module Detailed Functions:

★Image Display:Includes zoom in,zoom out,move image,move layer;

★Tool Selection:Circle,rectangle,polygon;

★One-Click Statistics:Quick count,sensitivity adjustment;

★Manual Correction:Click count,count undo,click to delete colony;

★Quick Functions:Restore original image,save,export PDF,export Excel;

★Total Colony Count Display:Measured area,actual colony count,plate inner diameter,colony count converted by inner diameter,sample dilution factor,colony count displayed by dilution factor;

★Colony Morphology Display:ID,position(coordinates of each counted colony),equivalent diameter,perimeter;

★Categorized Statistics Display:Serial number,size range,actual colony count;

★Instrument settings:Basic settings,Advanced settings,Lighting settings;

★Basic settings:Small colonies,Medium colonies,Large colonies,ROI,Colony outline;

★Advanced settings:Maximum colony diameter,Minimum colony diameter,Block size,Pixel ratio,Large/medium colony threshold,Small/medium colony threshold,Sample dilution factor,Plate diameter;

★Instrument Maintenance:Calibration;

(9)Total Bacteria Count-Membrane Filter Method Module Detailed Functions:

★Image Display:Includes zoom in,zoom out,move image,move layer;

★Tool Selection:Circle,Rectangle,Polygon;

★One-Click Statistics:Transverse counting,sensitivity adjustment;

★Manual Correction:Single-click counting,count undo,single-click colony deletion;

★Quick functions:Restore original image,save,export PDF,export Excel;

★Total colony count statistics display:Measured area,measured colony count,inner diameter of plate,colony count converted by inner diameter,sample dilution factor,colony count displayed by dilution factor;

★Colony morphology display:ID,position(coordinates of each counted colony),equivalent diameter,perimeter;

★Classification Statistics Display:Serial number,size range,measured colony count;

★Instrument Settings:Basic settings,advanced settings,lighting settings;

★Basic Settings:Small colonies,medium colonies,large colonies,ROI,colony outline;

★Advanced Settings:Maximum colony diameter,minimum colony diameter,block size,pixel ratio,large/medium colony threshold,small/medium colony threshold,sample dilution factor,plate diameter;

★Instrument Maintenance:Calibration;

(3)Database Security Management

Data Storage:Save information including sample name,sample ID,inoculation type(including inoculation volume,inoculation amount,dilution factor),test items,etc.

Smart Query:Quickly retrieve data by clicking year,month,or day.Alternatively,search by data name for rapid retrieval.

Data Export:Statistical results can be exported as PDF or Excel files.

Data Management:Features a four-tier architecture—“Operation,Review,Management,System”—with distinct roles and permissions to ensure data security,integrity,and authenticity.

Audit Trail:System administrators can view historical records of operator login times,personnel,operation names,and report titles.Audit trail results are exportable.

(4)*Report Generation:Includes institution name,project name,tester,testing time,method name,method standard number,incubation temperature,and incubation time.Positive Colony Count Analysis Results include:serial number,sample name,sample ID,test item,inoculum volume,unit,plate type,large-well positives,small-well positives,MPN,95%confidence interval,MPN/100mL,MPN/L.Colony Analysis Results include:serial number,account ID,sample name,sample ID,inoculum volume,unit,colony count.

4.Hardware Configuration

(1)Digital Imaging System Configuration:★6.3-megapixel CMOS camera featuring a 1/1.8"Sony Exmor back-illuminated CMOS sensor with dual-layer noise reduction technology.Integrated with 12-bit Ultra-fine™HISPVP and equipped with a USB 3.0 high-speed interface.5MP high-resolution lens;

(2)Main Unit:Dimensions(W×L×H):447mm×430mm×412mm;External Power Supply:AC220V 50Hz

(3)Screen Size:13.3-inch capacitive touchscreen all-in-one display

(4)Operating System:Genuine Windows 10 Pro

(5)Computer Hardware:i7 processor/8GB RAM/512GB SSD

(6)Software:1 set;