Washed red blood cells (WRBC) is an experimental procedure commonly used in surgery for transfusion of component blood products. It is the removal of all plasma and 90% of white blood cells and platelets from healthy blood. It is used clinically in anemic patients who have developed antibodies to leukocytes due to multiple transfusions and in patients after organ transplantation to reduce rejection reactions.

The whole blood is separated by centrifuge under aseptic conditions to firstly separate out the plasma and remove the white blood cells, add sterile saline to the red blood cells and mix, then centrifuge to remove the remaining white blood cells, so repeatedly wash 3 times to finally remove more than 98% of the plasma, more than 90% of the white blood cells and platelets, and also remove the metabolites such as potassium, ammonia and lactic acid produced during the preservation process, retaining more than 70% of the red blood cells. Finally, the addition of saline suspension is sufficient.


The purpose of this experiment is to solve the following problems: patients with allergic reactions (urticaria, angioneurotic edema, anaphylaxis, etc.) after whole blood or plasma transfusion; patients with autoimmune hemolytic anemia and paroxysmal sleep hemoglobinuria requiring transfusion; patients with hyperkalemia and liver and kidney dysfunction requiring transfusion; patients who have developed antibodies to leukocytes or platelets due to repeated blood transfusions Patients with febrile reactions.